Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons

Microelectrode surfaces covered with nanostructures derived from components of extracellular matrix, such as collagen fibers, have shown immense beneficial effects in promoting neuronal growth and cellular signaling. Synthetic mimicking the features biological durable conductive materials could promote cell adhesion on microelectrode by providing topographical cues simultaneously improve charge transfer properties reducing its global impedance. Therefore, an advanced nanostructuring method structural organizational natural fibers onto metallic has been presented here, which is adapted previous technological achievements group based nanoimprint lithography gold electroplating. Surface characterization methods reveal increase surface area between 20% 68% microelectrodes fabricated two different nanostructure heights. Impedance spectroscopy measurements reduction impedance magnitude (at 1 kHz) 22% 41%, depending upon height density microelectrode, should subsequently modulate for biosensing application. Cell analysis performed seal reveals a tighter coupling enteric neurons nanostructured microelectrodes. Finally, recordings exhibit significant improvement spike detection